Prevalence of Methicillin Resistant Staphylococcus Aureus in Various Clinical Specimen at KIST Medical College and Teaching Hospital

Abstract

Introduction: Antimicrobial resistance is a global threat and there is increase in antibiotic resistance in S.aureus. Multi-drug resistance developed in S. aureus has been associated with an increase in morbidity and mortality of the patients in the hospital. The main objective of this study was to detect MRSA using phenotypic methods and to determine their antibiogram.

Methods: Various specimens received from September 2020 to september 2021 in Kist Medical College-Teaching Hospital were processed and all S. aureus isolates were included in the study.

The isolates were identified by standard laboratory procedure. Cefoxitin was used to detect MRSA by the disk diffusion test. The antibiotic susceptibility pattern of all isolates was determined by modified Kirby Bauer disc diffusion method.

Results: A total of 5,198 samples (blood 1683, pus 130, swab 328, body fluid 849, urine 2208) from patients attending the Kist hospital for treatment were collected. From the total 5,198 samples 80 were confirmed as Staphylococcus aureus. Out of 80 S.aureus isolated 49 (61.3%) were found to be MRSA. All the S.aureus isolated was sensitive to vancomycin. Among 49 MRSA isolates 33 (67.34%) were MDR and among 31 MSSA isolates 4 (12.9%) were MDR.

Conclusion: This study showed a high prevalence of MRSA in tertiary care hospital. Regular surveillance of healthcare-associated infection and monitoring of antibiotic sensitivity pattern is mandatory to reduce MRSA prevalence in hospital. Present study shows that vancomycin remains the drug of choice for MRSA infection.